Today I Exosap'd Exons 8 and 9 and transferred them to plates. I made sure to orient the DNA samples in such a way that it would speed up the process of loading them into the trays for sequencing. Any little thing to speed up the process helps! I also did an amplification on Exon 10, the next exon in line to be sequenced. In addition, I ran a second amplification on Exon 7. In my last gel the primer dimer was VERY bright. Too bright compared to the DNA band. I added less primer to this amplification, so hopefully it will be more successful.
Since I am at a break of sorts (while two of the PCR machines are in use) I am going to the Student Athlete/Artist exhibit in the Student Life center. When I get back I will run the two amplifications on a gel and try to amplify Exon 11 and 12. If the gel turns out alright I will probably start tomorrow off with exosaping Exons 7 and 10. I'm glad I'm starting to move through the Exons quicker . . . although I'm still not sure if I'll be able to finish everything in time! If I can't then my presentation and paper will just have to be about the process of working between labs, the steps I took to sequence what I could, and any results I acquire.
Speaking of results, the only base change(s) I found from Exon 1 and 2 are insignificant. Melanie emailed me; she will explain how she came to that conclusion next time I go to the center. The more experience I can get with this type of research, the better!
No comments:
Post a Comment