Good news . . . bottle has been opened.

I got one of my friends to open it for me.  It was really frustrating to have something so simple halt my work, especially since I have been working long hours and nonstop to try to get all of the work done.  I don't really have time to spare.

I made a new gel and buffer solution before lunch so that the gel could solidify.  I loaded the gel with some samples from Exons 6 through 9 and I'm running it now.  In the meantime I consolidated my tubes since I am running low on boxes to put the tubes in, AGAIN.  I also counted out and numbered tubes for the next two Exosap PCRs I'm going to run.

I'm only running two PCRs at a time because I haven't tried the third machine and at this point in the game it would be just as efficient to run two PCRs as it would be to run three.  That way, if the third PCR machine here isn't working properly I can avoid wasting time and PCR ingredients.  Working in the lab is all about efficiency right now!  I also have to make a list of what to bring back from the GGC next.  I'm running low on dNTPs and ExoSap and well . . . pretty much everything except the polymerase buffer.  I've got plenty of that!  I'll let you know how my gel turns out in a little bit.