I went to the center today, and it was quite the busy day. For the first part of the day, I got all of the remaining exons ready to be sequenced up to the BigDyeFast step. By 3:00 I had submitted four plates of samples for sequencing. Tomorrow I will submit the last four. I also analyzed four sequences while I was at the center. This took the last couple of hours that I was at the center.
When analyzing sequences I compare 10-15 of the samples to the regular transcript of the gene, and anything that doesn't match the transcript shows up in red. Most of the time it's an "ambiguity code" like a Y or a W, and you have to look at the colors of the wave signals to see what the base is. If it's not an ambiguity code and you see a base change, you still probably need to check the waves to make sure the sample isn't messy looking.
If an SNP is found, I think the next step would be to screen the "normal population" DNA plates. Hopefully I'm able to locate some sort of mutation within this gene!
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