Today I realized how much quicker my pace is now that I'm kind of in the swing of things. I only have Exons 13 and 14 to amplify, run a gel on and exosap. I have to exosap exon 7 (the first try, since the second amplification with less primer didn't even work), exon 10, and exon 12.
I somehow forgot to exosap exon 10 and moved straight to 11. I guess that's what having a multitude of tiny tubes does to you. I've decided to make use of the white board and start writing what I have left to do so I can cross off tasks as they are completed.
My parents might visit me soon so that I can show them my lab. I hope they like it! I feel like such a big girl working in here by myself with all this DNA.
Worrisome notification of the day: I'm running out of 10 microliter pipette tips. I basically need 70 more since I can use the other pipette tips for everything except the initial amplification. Thirty-five patients multiplied by 2 exons = 70 tips needed! I've rummaged through the lab I'm in and the supply room, but I think I'm going to have to ask one of the biology professors tomorrow. For today I'll try to finish exosap'ing what I have amplified and run on gels.
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